The Institute of Human Genetics (Institut de Génétique Humaine, IGH) is a major CNRS unit dedicated to excellence in fundamental research and pathology. The principal research interests of the IGH are genome dynamics, developmental genetics, epigenetics, and molecular and cellular pathology.
Seminar Series on Molecular Bases of Human Diseases
Amphitheater Genopolys, at 11:30, from October 16th to May 19th
Seminars of the month @ IGH
02-02-2016 (14h00) "Post-doc Interview: Functional Characterization of p53-Induced Noncoding Transcript (Lincpint)" Oskar MARIN(Huarte Lab)
The p53 transcription factor regulates a complex transcriptional network that is crucial to maintain cellular homeostasis. Only recently it has become clear that p53 regulates the expression of several long intergenic noncoding RNAs (lincRNAs). One of them, named Lincpint (p53 induced noncoding transcript), is the subject of this study.
Lincpint is a direct transcriptional target of p53. In mouse cells, Lincpint promotes cell proliferation and survival by regulating the expression of genes of the TGF-β, MAPK and p53 pathways. Lincpint is a nuclear lincRNA that directly interacts with the Polycomb repressive complex 2 (PRC2), and is required for PRC2 targeting of specific genes for H3K27 tri-methylation and repression. Murine Lincpint has an ortholog in human (LINC-PINT), which presents suggestive analogies with the murine lincRNA, such as its regulation by p53. Its expression significantly correlates with the same cellular pathways as the mouse ortholog, including the p53 pathway. Interestingly, LINC-PINT is downregulated multiple tumor types. Its overexpression inhibits the proliferation, in vivo tumor growth and migration-invasion capability of tumor cells. Altogether, these data suggest a possible role of LINC-PINT as tumor suppressor.
19-02-2016 (11h30) "Temporal regulation of genome replication" Conrad A. NIEDUSZYNSKI(Sir William Dunn School of Pathology, Oxford, UK)
Some recent papers
Menolfi D, Delamarre A, Lengronne A, Pasero P, Branzei D. "Essential Roles of the Smc5/6 Complex in Replication through Natural Pausing Sites and Endogenous DNA Damage Tolerance" (2015), Mol. Cell: 60(6):835-46
Rojas-Rios, P., Chartier, A., Pierson, S., Severac, D., Dantec, C., Busseau, I., Simonelig, M. "Translational Control of Autophagy by Orb in the Drosophila Germline" (2015), Developmental Cell: 35, 5, 622-631
Cayrou C, Ballester B, Peiffer I, Fenouil R, Coulombe P, Andrau JC, van Helden J, Méchali M. "The chromatin environment shapes DNA replication origin organization and defines origin classes" (2015), Genome Res: 25(12):1873-85
Traver S, Coulombe P, Peiffer I, Hutchins JR, Kitzmann M, Latreille D, Méchali M "MCM9 Is Required for Mammalian DNA Mismatch Repair" (2015), Mol Cell: 59(5):831-839
Barckmann,B., Pierson,S., Dufourt, J., Papin, C., Armenise, C., Port, F., Grentzinger, T., Chambeyron, S., Baronian, G., Desvignes, JP., Curk, T., Simonelig, M. "Aubergine iCLIP Reveals piRNA-Dependent Decay of mRNAs Involved in Germ Cell Development in the Early Embryo" (2015), Cell Rep: 12(7):1205-1216
Kermi, C., Prieto, S., van der Laan, S., Tsanov, N., Recolin, B., Uro-Coste, E., Delisle, M-B., and Maiorano, D. "Rad18 is a maternal limiting factor that suppresses the UV-dependent DNA damge checkpoint in Xenopus embryos" (2015), Developmental Cell : 34(3):364-372
Reynaud E, Lahaye LL, Boulanger A, Petrova IM, Marquilly C, Flandre A, Martianez T, Privat M, Noordermeer JN, Fradkin LG, Dura JM "Guidance of Drosophila Mushroom Body Axons Depends upon DRL-Wnt Receptor Cleavage in the Brain Dorsomedial Lineage Precursors" (2015), Cell Rep: 11(8):1293-304
Gonzalez I, Munita R, Agirre E, Dittmer TA, Gysling K, Misteli T, Luco RF. "A lncRNA regulates alternative splicing via establishment of a splicing-specific chromatin signature" (2015), Nat Struct Mol Biol: 22, 5, 370-376
Fragkos M, Ganier O, Coulombe P, Méchali M "DNA replication origin activation in space and time" (2015), Nat Rev Mol Cell Biol: 16(6):360-74
Ide, S., Dejardin, J. "End-targeting proteomics of isolated chromatin segments of a mammalian ribosomal RNA gene promoter" (2015), Nat. Commun.: 6, 6674
Borde V, de Massy B "Meiosis: early DNA double-strand breaks pave the way for inter-homolog repair" (2015), Dev Cell: 32(6):663-4
Sexton, T., Cavalli, G. "The Role of Chromosome Domains in Shaping the Functional Genome" (2015), CELL: 160, 6, 1049–1059
Marzec, P., Armenise, C., Perot, G., Roumelioti, F.M., Basyuk, E., Gagos, S., Chibon, F., Dejardin, J. "Nuclear-Receptor-Mediated Telomere Insertion Leads to Genome Instability in ALT Cancer" (2015), CELL: 160, 5, 913-927
Seminar series on Molecular Bases of Human diseases: Recognition of DNA by the innate immune system 14-03-2016, Søren RIIS PALUDAN (Department of Biomedicine, Aarhus University, Denmark)
Seminar series on Molecular Bases of Human diseases: Endogenous DNA damage and the production of blood - a tale of two aldehydes 01-04-2016, Ketan J. PATEL (Laboratory of Molecular Biology, Cambridge, UK)
Seminar series on Molecular Bases of Human diseases: DNA replication stress and cancer 22-04-2016, Tano HALAZONETIS (University of Geneva, Switzerland)
Seminar series on Molecular Bases of Human diseases: The Road to Utopia: Challenges in linking literature and research data 19-05-2016, Terri ATTWOOD (Life Sciences and School of Computer ScienceThe University of Manchester, UK)
Building neural circuits interconnecting the left and right sides of the central nervous system: molecular mechanisms of midline crossing 03-06-2016, Valérie Castellani (INMG - Université de lyon1)
Multiple functions of piRNAs and PIWI proteins 06-04-2016, Montpellier, France
ICTE2016: International Congress on Transposable Elements 16-04-2016, Saint-Malo, France
Poli J, Gerhold CB, Tosi A, Hustedt N, Seeber A, Sack R, Herzog F, Pasero P, Shimada K, Hopfner KP, Gasser SM.. Mec1, INO80, and the PAF1 complex cooperate to limit transcription replication conflicts through RNAPII removal during replication stress (2016), Genes Dev.. Lub S, Maes A, Maes K, De Veirman K, De Bruyne E, Menu E, Fostier K, Kassambara A, Moreaux J, Hose D, Leleu X, King RW, Vanderkerken K, Van Valckenborgh E.. Inhibiting the anaphase promoting complex/cyclosome induces a metaphase arrest and cell death in multiple myeloma cells (2016), Oncotarget..