Contact : Reini FERNANDEZ DE LUCO
The role of lncRNAs in the epithelial-to-mesenchymal transition.
The epithelial-to-mesenchymal transition (EMT) is a biological process that plays an essential role in pathways such as embryonic development and wound healing. However, it also plays a pivotal part during tumorigenesis, allowing tumour epithelial cells to lose their polarity and their capacity to form inter-cellular junctions, and in this manner gaining a strong capacity for migration. Carcinoma EMT is one of the first steps leading to metastasis and contributes to a highly unfavourable prognostic, giving the tumour a larger adaptation capacity, including to chemotherapy. Recently, several studies have demonstrated the potential implication of long non-coding RNAs (lncRNAs) in EMT progression. Furthermore, preliminary genome-wide sequencing data obtained in the host lab, have allowed us to identify several lncRNAs which not only change expression during EMT but are also neighbouring genes whose correct expression is essential for this detrimental cellular transition to occur and whose expression are also changing, making these lncRNAs prime candidates in EMT regulation.
Using next-generation sequencing data obtained from cells at various stages of the EMT process, molecular biology techniques and human mammary epithelial cell culture, the goal of the student will be to identify and characterize lncRNAs playing an essential role in the regulation of genes that control the EMT process.
The internship will take place in Reini Luco’s laboratory at the Institute of Human Genetics in Montpellier under the supervision of Andrew Oldfield.
Post Doctoral Position
Contact : Martine SIMONELIG
Functions of the piRNA pathway in germline stem cells
A two-year post-doctoral position funded by the Labex EpiGenMed is available in the group mRNA Regulation and Development at the Institute of Human Genetics in Montpellier. Our lab works on gene regulation at the mRNA level during development and diseases, in the Drosophila model. We have described the role of Piwi-interacting RNAs (piRNAs), a specific class of small non-coding RNAs, in gene regulation for several developmental processes. This function was unexpected because piRNAs were thought to be specific to the regulation of transposable elements. piRNAs and PIWI proteins are highly expressed in germ cells and stem cell lineages. The post-doctoral project aims at understanding the functions of the piRNA pathway in stem cells, using germline stem cells as a model system.
The Institute of Human Genetics provides a very stimulating and international research environment; it is fully endowed with state-of-the-art scientific equipment. The Institute is located in Montpellier, an international city in the South of France. http://www.igh.cnrs.fr/
We are looking for very motivated and ambitious candidates with strong expertise in Drosophila genetics, imaging, metabolism, or RNA biology.
Candidates should apply by sending a CV, a short outline of current projects, and contact information of two to three references to Martine Simonelig. Starting date of the contract: October 1st, 2017.
Selected publications of the lab, related to the project:
Rojas Ríos P*, Chartier A*, Pierson S, Séverac D, Dantec C, Busseau I, Simonelig M. Translational control of autophagy by Orb in the Drosophila germline (2015) Developmental Cell, 35, 622-631
Barckmann B, Pierson S, Dufourt J, Papin C, Armenise C, Port F, Grentzinger T, Chambeyron S, Baronian G, Desvignes JP, Curk T, Simonelig M. Aubergine iCLIP reveals piRNA-dependent decay of mRNAs involved in germ cell development in the early embryo (2015) Cell Reports,12,1205-16
Simonelig M. piRNAs, master regulators of gene expression (2014) Cell Research, 24, 779-80
Joly W, Chartier A, Rojas-Rios P, Busseau I, Simonelig M. The CCR4 deadenylase acts with Nanos and Pumilio in the fine-tuning of Mei-P26 expression to promote germline stem cell self-renewal (2013) Stem Cell Reports, 1, 411-424
Rouget C, Papin C, Boureux A, Meunier A-C, Franco B, Robine N, Lai EC., Pélisson A, Simonelig M. Maternal mRNA deadenylation and decay by the piRNA pathway in the early Drosophila embryo (2010) Nature, 467, 1128-1132
Contact: Martine Simonelig
mRNA Regulation and Development
Department Genetics and Development
Institute of Human Genetics, Montpellier, France