(offre N° 78)
Contact : Martine SIMONELIG
Functions of the piRNA pathway in germline stem cells
A two-year post-doctoral position funded by the Labex EpiGenMed is available in the group mRNA Regulation and Development at the Institute of Human Genetics in Montpellier. Our lab works on gene regulation at the mRNA level during development and diseases, in the Drosophila model. We have described the role of Piwi-interacting RNAs (piRNAs), a specific class of small non-coding RNAs, in gene regulation for several developmental processes. This function was unexpected because piRNAs were thought to be specific to the regulation of transposable elements. piRNAs and PIWI proteins are highly expressed in germ cells and stem cell lineages. The post-doctoral project aims at understanding the functions of the piRNA pathway in stem cells, using germline stem cells as a model system.
The Institute of Human Genetics provides a very stimulating and international research environment; it is fully endowed with state-of-the-art scientific equipment. The Institute is located in Montpellier, an international city in the South of France. http://www.igh.cnrs.fr/
We are looking for very motivated and ambitious candidates with strong expertise in Drosophila genetics, imaging, metabolism, or RNA biology.
Candidates should apply by sending a CV, a short outline of current projects, and contact information of two to three references to Martine Simonelig. Starting date of the contract: October 1st, 2017.
Selected publications of the lab, related to the project:
Rojas Ríos P*, Chartier A*, Pierson S, Séverac D, Dantec C, Busseau I, Simonelig M. Translational control of autophagy by Orb in the Drosophila germline (2015) Developmental Cell, 35, 622-631
Barckmann B, Pierson S, Dufourt J, Papin C, Armenise C, Port F, Grentzinger T, Chambeyron S, Baronian G, Desvignes JP, Curk T, Simonelig M. Aubergine iCLIP reveals piRNA-dependent decay of mRNAs involved in germ cell development in the early embryo (2015) Cell Reports,12,1205-16
Simonelig M. piRNAs, master regulators of gene expression (2014) Cell Research, 24, 779-80
Joly W, Chartier A, Rojas-Rios P, Busseau I, Simonelig M. The CCR4 deadenylase acts with Nanos and Pumilio in the fine-tuning of Mei-P26 expression to promote germline stem cell self-renewal (2013) Stem Cell Reports, 1, 411-424
Rouget C, Papin C, Boureux A, Meunier A-C, Franco B, Robine N, Lai EC., Pélisson A, Simonelig M. Maternal mRNA deadenylation and decay by the piRNA pathway in the early Drosophila embryo (2010) Nature, 467, 1128-1132
Contact: Martine Simonelig
mRNA Regulation and Development
Department Genetics and Development
Institute of Human Genetics, Montpellier, France
(offre N° 77)
Contact : Marcel MECHALI
Postdoctoral position on DNA replication
Place of work
A postdoctoral position is open in our laboratory, DNA Replication and Genome Dynamics, located at the Institute of Human Genetics, a modern large CNRS-University Institute located in the University and Hospital campus of Montpellier. Fully endowed with state-of-the-art scientific equipment, the IGH generates a stimulating and international research environment, for high-quality and intensive scientific life. The working language is English. The city is one of the most lively and concentrated areas for research scientists in France.
Our multinational team wishes to understand how DNA replication initiation is regulated, how replication origins are recognized, and how this process is linked to genome dynamics. In recent years, our laboratory unravelled how genetic and epigenetic features shape DNA replication origin recognition and organization. The projects focus on genetic and epigenetic aspects of DNA replication, and investigations on the mechanism of origin recognition at the protein level. Our methods use cell culture, in-vitro replication systems, Next-Generation Sequencing coupled to bioinformatics analyses, proteomics, and a wide range of molecular and cell biology methods.
Some Recent publications
• Rodríguez-Martínez M, Pinzón N, Ghommidh C, Beyne E, Seitz H, Cayrou C, and Méchali M. (2017) The gastrula transition reorganizes replication-origin selection in Caenorhabditis elegans. Nat Struct Mol Biol. 24, 290-299.
• Fragkos, M., Ganier, O., Coulombe, P. and Méchali, M. (2015) DNA replication origin activation in space and time. Nature Rev. Mol. Cell Biol. 16, 360-374.
• Cayrou C, Ballester B, Peiffer I, Fenouil R, Coulombe P, Andrau JC, van Helden J, Méchali M. (2015), The chromatin environment shapes DNA replication origin organization and defines origin classes. Genome Res., 12, 1873-1885.
• Traver, S., Coulombe, P., Kitzmann, M., Peiffer, I., Hutchins, J., Latreille, D., and Méchali, M. (2014) MCM9 is Required for Mammalian DNA Mismatch Repair. Mol. Cell, 59, 831-839.
• Coulombe, P., Grégoire, D., Tsanov, N., and Méchali, M. (2013) A spontaneous Cdt1 mutation in 129 mouse strains reveals a regulatory domain restraining replication licensing. Nature Commun. 4, 1-10.
• Lutzmann, M., Grey, C., Traver, S., Ganier, O., Maya-Mendoza, A., Ranisavljevic, N., Bernex, F., Nishiyama, A., Montel, N., Gavois, E., Forichon, L., de Massy, B., and Méchali, M. (2012) MCM8- and MCM9-Deficient Mice Reveal Gametogenesis Defects and Genome Instability Due to Impaired Homologous Recombination. Mol Cell. 47, 523-534.
We would be glad to receive applications from highly motivated scientists, able to work independently, with a solid background in nuclear metabolism and strong expertise in molecular biology. We are also interested in applicants with a double background in bioinformatics and molecular biology. The position is for at least two years.
Please submit a curriculum vitae and contact information of two referees to Marcel Méchali, Institute of Human Genetics, 141 rue de la Cardonille, 34396 Montpellier, France.
Tel: +33 (0) 434 359 917; e-mail: email@example.com