Cavalli lab - Lab Goodies
This is the download section of
"certified lab protocols" (i.e. the stuff you are supposed to be able
to do without major problems if you follow the protocol precisely). Try
them out, they should work! Of course bugs are always waiting behind
the corner. If you find them, please let us know by email,
so we can correct them for the others out there.
Polytene chromosome protocols
Protocols on polytene chromosome immunostaining, or for combinations of immunostaining and DNA Fluorescent In Situ hybrization (FISH)
1) Immunostaining of polytene chromosomes - PDF format (40 Kb)
...or combine Immunostaining with DNA FISH:
2) Immunostaining of polytenes followed by DNA in situ fluorescent hybridization - PDF format (1.1 Mb). This protocol also contains comments and troubleshooting section. It has been published. Its citation is: Lavrov, S., Déjardin, J., and Cavalli, G. (2004). Combined immunostaining and FISH analysis of polytene chromosomes. Methods Mol Biol 247, 289-303.
... or do it the other way around:
3) In situ fluorescent hybridization followed by immunostaining. This protocol contains a discussion on when and why try first FISH and then Immuno- or vice versa. The manuscript has been reviewed, edited and published online on February 2005 by the European Union Network of Excellence "The Epigenome". This protocol can be found in the "Research tools" section of the Epigenome website.
Two-colour fluorescent in situ DNA hybridization on whole mount Drosophila embryos and larval imaginal discsThis protocol contains a step-by-step procedure for in situ DNA hybridization of different types of Drosophila tissues. The protocol has been reviewed and edited by the European Union Network of Excellence "The Epigenome", and it was published online on June 2005. This protocol can be found in the "Research tools" section of the Epigenome website.
Go to the online version of the protocol!
or you can download a pdf version of this protocol by clicking here.
|Chromatin Immunoprecipitation in Drosophila tissues
Download a protocol for formaldehyde cross-linking/chromatin immunoprecipitation (ChIP) in Drosophila embryos - PDF format
Same as above, the protocol has detailed instructions and a troubleshooting section. It has been published and its bibliographic citation is: Cavalli, G., Orlando, V., and Paro, R. (1999). Mapping DNA target sites of chromatin-associated proteins by formaldehyde cross-linking in Drosophila embryos. In: Chromosome Structural Analysis: A Practical Approach, pp20-37. Ed. W. A. Bickmore. Oxford University Press. UK.
Download a detailed protocol for Transgenesis in Drosophila - PDF format (460 Kb)
This protocol contains detailed instructions and a troubleshooting section, schemes and pictures of all critical steps of the injection procedure, and also a description of the crosses one needs to do to establish and characterize stable transgenic lines once the transrformants have been obtained
Last update: 07/01/2009