PIWI proteins and PIWI-interacting RNAs (piRNAs) are essential for silencing mobile genetic elements like transposons in the animal germline and are essential for fertility in animals and humans. This meeting aims to bring the community to discuss the recent advances in the field and will cover a broad range of techniques and animal models. Speakers at this meeting will illustrate the unprecedented molecular insights into piRNA biogenesis machinery provided by structural biology investigations and explore the use of new animal model systems. While the role of piRNAs in gene regulation within the germline and in soma is now firmly established, the molecular insights are only starting to be uncovered. We will provide ample opportunities for young scientists in our community and aim to share mostly latest unpublished data. This meeting will foster new collaborations across model systems and exchange of reagents and tools.