Biology of Repetitive Sequences

  • Chromatin can be viewed as a highly complex mixture of proteins and nucleic acids that orchestrate DNA-based processes in the eukaryotic genome. Most of the mammalian genome is assembled into heterochromatin, a ‘closed’ structure imposed by several enzymatic activities. Such activities act on histones and the DNA itself to impinge on transcription, replication or repair.
    Most of the heterochromatic fraction of the genome can be found at critical loci. These include telomeres, repetitive sequences around centromeres and a portion (about half) of the gene units encoding ribosomal RNAs. Defects in the regulation of these loci have therefore disastrous consequences on cell identity and can lead to developmental problems, cancer, premature aging or immune deficiencies. How precisely heterochromatic enzymes affect the composition of target loci has remained elusive and research in our laboratory primarily focuses on this question.
    To understand how heterochromatin acts at the molecular level, we are looking at the effect of abrogating important heterochromatic activities, such as histone and/or DNA methyl-transferases, on the overall composition of key heterochromatic loci (telomeres, pericentromeres and rDNA).


    Members

    Jérôme Dejardin
    Dejardin Jérôme
    Mathieu Tardat
    Tardat Mathieu
    Sihem Zitouni
    Zitouni Sihem
    Sandrine Sauzet
    Sauzet Sandrine
    Lucas Ducrot
    Ducrot Lucas
    Bariaa Hamade
    Hamade Bariaa
    Varinia Garcia molinero
    Garcia molinero Varinia
    Laëtitia Guintini
    Guintini Laëtitia
    Amandine Barral
    Barral Amandine
    Mathilde Gauchier
    Gauchier Mathilde

    Publications

    SMCHD1 is involved in de novo methylation of the DUX4-encoding D4Z4 macrosatellite.

    Dion C, Roche S, Laberthonnière C, Broucqsault N, Mariot V, Xue S, Gurzau AD, Nowak A, Gordon CT, Gaillard MC, El-Yazidi C, Thomas M, Schlupp-Robaglia A, Missirian C, Malan V, Ratbi L, Sefiani A, Wollnik B, Binetruy B, Salort Campana E, Attarian S, Bernard R, Nguyen K, Amiel J, Dumonceaux J, Murphy JM, Déjardin J, Blewitt ME, Reversade B, Robin JD, Magdinier F

    2019 - Nucleic Acids Res

    Request for full article30698748

    Integrative Proteomic Profiling Reveals PRC2-Dependent Epigenetic Crosstalk Maintains Ground-State Pluripotency.

    van Mierlo G, Dirks RAM, De Clerck L, Brinkman AB, Huth M, Kloet SL, Saksouk N, Kroeze LI, Willems S, Farlik M, Bock C, Jansen JH, Deforce D, Vermeulen M, Déjardin J, Dhaenens M, Marks H

    2018 - Cell Stem Cell, 24(1):123-137

    Request for full article30472157

    Telomere chromatin establishment and its maintenance during mammalian development.

    Tardat M, Déjardin J

    2017 - Chromosoma, 127(1):3-18

    Request for full article29250704

    The molecular basis of the organization of repetitive DNA-containing constitutive heterochromatin in mammals

    Nishibuchi G, Déjardin J.

    2017 - Chromosome Res., 25(1):77-87

    Request for full article28078514
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