Biology of Repetitive Sequences

  • Chromatin can be viewed as a highly complex mixture of proteins and nucleic acids that orchestrate DNA-based processes in the eukaryotic genome. Most of the mammalian genome is assembled into heterochromatin, a ‘closed’ structure imposed by several enzymatic activities. Such activities act on histones and the DNA itself to impinge on transcription, replication or repair.
    Most of the heterochromatic fraction of the genome can be found at critical loci. These include telomeres, repetitive sequences around centromeres and a portion (about half) of the gene units encoding ribosomal RNAs. Defects in the regulation of these loci have therefore disastrous consequences on cell identity and can lead to developmental problems, cancer, premature aging or immune deficiencies. How precisely heterochromatic enzymes affect the composition of target loci has remained elusive and research in our laboratory primarily focuses on this question.
    To understand how heterochromatin acts at the molecular level, we are looking at the effect of abrogating important heterochromatic activities, such as histone and/or DNA methyl-transferases, on the overall composition of key heterochromatic loci (telomeres, pericentromeres and rDNA).


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    Members

    Jérôme Dejardin
    Dejardin Jérôme
    Mathieu Tardat
    Tardat Mathieu
    Sihem Zitouni
    Zitouni Sihem
    Sandrine Sauzet
    Sauzet Sandrine
    Assia Bouhayane
    Bouhayane Assia
    Lucas Ducrot
    Ducrot Lucas
    Bariaa Hamade
    Hamade Bariaa
    Varinia Garcia molinero
    Garcia molinero Varinia
    Amandine Barral
    Barral Amandine

    Publications

    Telomeric Chromatin and TERRA.

    Barral A, Déjardin J

    2020 - J Mol Biol

    Request for full article32151584

    Purification and enrichment of specific chromatin loci.

    Gauchier M, van Mierlo G, Vermeulen M, Déjardin J

    2020 - Nat Methods, 17(4):380-389

    Request for full article32152500

    Locus-specific chromatin isolation.

    Vermeulen M, Déjardin J

    2020 - Nat Rev Mol Cell Biol, 21(5):249-250

    Request for full article31996790

    Remodeling and destabilization of chromosome 1 pericentromeric heterochromatin by SSX proteins.

    Traynor S, Møllegaard NE, Jørgensen MG, Brückmann NH, Pedersen CB, Terp MG, Johansen S, Dejardin J, Ditzel HJ, Gjerstorff MF

    2019 - Nucleic Acids Res, 47(13):6668-6684

    Request for full article31114908
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