Genome Surveillance and Stability

Molecular bases of human diseases

Our group is generally interested in the regulation of the DNA Damage Response (DDR), and in particular in its regulation during the early steps of embryonic development. The main function of the DDR is to slow down, or arrest cell prolferation in the presence of DNA lesions (DNA breaks, telomer integrity, replication forks stall) so to avoid cell division in the presence of DNA damage and therefore avoid the propagation of mutations that are drivers of genomic instability. A strong genomic instability is a feature of cancer cells (telomeric fusions, trabnsocations, duplications, deletions). To date it is admitted that the majority of sporadic tumours are the consequence of mutations in DDR genes, which can also lead to the development of genetic diseases. Hence the DDR is currently considered as a major barrier to malignat transformation playing a key role in maintenace of genomic stability.

 

Antoine AZE
Researcher

0434359973
James Hutchins
James HUTCHINS
Researcher

0434359973
Domenico MAIORANO
Researcher

0434359946 / 0434359973
Cyril RIBEYRE
Researcher

0434359815
Nathalie Malirat
Nathalie MALIRAT
IT

0434359973
Nour Benbahouche
Nour BENBAHOUCHE
Postdoc

0434359973
Hanane Mechri
Hanane MECHRI
PhD Student

0434359973
Sibylle Bellone
Sibylle BELLONE
Stagiaire

PUBLICATIONS OF THE TEAM

Studying the DNA damage response in embryonic systems.

Lo Furno E, Recolin B, van der Laan S, Aze A, Maiorano D

Translesion DNA synthesis-driven mutagenesis in very early embryogenesis of fast cleaving embryos

Lo Furno E., Busseau, I., Aze, A., Lorenzi, C., Saghira, C., Danzi, M., Zuchner, S., Maiorano, D.

more

Translesion Synthesis or Repair by Specialized DNA Polymerases Limits Excessive Genomic Instability upon Replication Stress.

Maiorano D, El Etri J, Franchet C, Hoffmann JS

Dihydropyrimidinase protects from DNA replication stress caused by cytotoxic metabolites

Jihane Basbous, Antoine Aze, Laurent Chaloin, Rana Lebdy, Dana Hodroj, Cyril Ribeyre, Marion Larroque, Caitlin Shepard, Baek Kim, Alain Pruvost, Jérôme Moreaux, Domenico Maiorano, Marcel Mechali, Angelos Constantinou

more

Preserving Genome Integrity During the Early Embryonic DNA Replication Cycles.

Kermi C, Aze A, Maiorano D

more

Recent advances in understanding DNA replication: cell type-specific adaptation of the DNA replication program.

Aze A, Maiorano D

+ Replication and Genome Dynamics

Reversible p53 inhibition prevents cisplatin ototoxicity without blocking chemotherapeutic efficacy

Benkafadar N, Menardo J, Bourien J, Nouvian R, François F, Decaudin D, Maiorano D, Puel JL, Wang J.

Regulation of DNA Replication in Early Embryonic Cleavages

Kermi, C., Lo Furno, E., Maiorano, D

An ATR-dependent function for the Ddx19 RNA helicase in nuclear R-loop metabolism

Hodroj D, Recolin B, Serhal K, Martinez S, Tsanov N, Abou Merhi R, Maiorano D

Ddx19 links mRNA nuclear export with progression of transcription and replication and suppresses genomic instability upon DNA damage in proliferating cells

Hodroj, D., Serhal, K., Maiorano, D.

Chapter 6 -Open Access - Genomic instability of pluripotent stem cells: origins and consequences

Lo Furno, E., van der Laan, S., and Maiorano, D.

Regulation of translesion DNA synthesis by PCNA monoubiquitylation and beyond

Tsanov, N., Kermi, C., Delgado, J., Serrano, L., Maiorano D.

Rad18 is a maternal limiting factor that suppresses the UV-dependent DNA damge checkpoint in Xenopus embryos

Kermi, C., Prieto, S., van der Laan, S., Tsanov, N., Recolin, B., Uro-Coste, E., Delisle, M-B., and Maiorano, D.

Checking the cycle by ERRβ splice variants

Vanacker JM, Maiorano D.

PIP degron proteins, substrates of CRL4Cdt2, and not PIP boxes, interfere with DNA polymerase h and k focus formation upon UV damage

Tsanov, N., Kermi, C., Coulombe, P., Van der Laan, S., Hodroj, D., Maiorano, D.

Post-translational modifications in embryonic cell cycle

Van der Laan, S., Maiorano, D.

Cell Cycle-Dependent Expression of Dub3, Nanog and the p160 Family of Nuclear Receptor Coactivators (NCoAs) in Mouse Embryonic Stem Cells

van der Laan S, Golfetto E, Vanacker JM, Maiorano D.

Molecular mechanisms of DNA replication checkpoint activation

Recolin B, van der Laan S, Tsanov N, Maiorano D.

DNA polymerase k-dependent DNA synthesis at stalled replication forks is important for Chk1 activation

Bétous R., Pillaire, M-J, Pierini, L., Van der Laan, S., Recolin B., Ohl-Séguy, E., Guo, C., Niimi, N., Gruz, P., Nohmi, T. Friedberg, E., Cazaux, C., Maiorano, D* and Hoffmann J-S*. * corresponding authors

Polk in replication checkpoint

Maiorano, D., Hoffmann, JS.

High Dub3 expression in mouse ESC couples the G1/S checkpoint to pluripotency

Van der Laan, S., Crozet, C., Tsanov, N., and Maiorano, D

Role of RPA as sensor in activation of the S-phase checkpoint in Xenopus egg extracts

Recolin, B., Van der Laan, S., and Maiorano, D.

Implication of RPA32 phosphorylation in S-phase checkpoint signalling at replication forks stalled with aphidicolin in Xenopus egg extracts

Recolin, B., Maiorano, D

XRCC1 interacts with the p58 subunit of DNA Pola-primase and may coordinate DNA repair and replication during S phase.

Levy N, Oehlmann M, Delalande F, Nasheuer HP, Van Dorsselaer A, Schreiber V, De Murcia G, Ménissier-de Murcia J, Maiorano D, Bresson Anne.

Regulation of S phase during early embryonic Xenopus development

Recolin, B., Maiorano, D.

Geminin is cleaved by caspase-3 during apoptosis in Xenopus egg extracts

Auziol C, Mechali M, Maiorano D.

+ Replication and Genome Dynamics

A Cdt1-geminin complex licenses chromatin for DNA replication and prevents rereplication during S phase in Xenopus.

Lutzmann M, Maiorano D, Mechali M.


+ Replication and Genome Dynamics

Recombinant Cdt1 induces rereplication of G2 nuclei in Xenopus egg extracts

Maiorano, D., Krasinska, L., Lutzmann, M. and Mechali M.


+ Replication and Genome Dynamics

MCM8, a novel DNA helicase which is not required for licensing but functions during processive chromosomal replication in vertebratres.

Maiorano, D., Cuvier, O., Danis, E., and Mechali, M.


+ Replication and Genome Dynamics

Identification of full genes and proteins of MCM9, a novel, vertebrate-specific member of the MCM2-8 protein family

Lutzmann, M., Maiorano, D., and Méchali, M.


+ Replication and Genome Dynamics

A hypophosphorylated form of RPA34 is a specific component of pre-replication centers.

Françon, P. ; Lemaitre, JM., Dreyer, C. ; Maiorano, D. ; Cuvier, O. and Marcel Méchali.


+ Replication and Genome Dynamics

Crystal Structure of the Coiled-coil Dimerization Motif of Geminin : Structural and Functional Insights on DNA Replication Regulation.

Thepaut, M., Maiorano, D., Guichou, JF., Auge, MT., Dumas, C., Méchali, M., and Padilla, A.


+ Replication and Genome Dynamics

Cell cycle regulation of the licensing activity of Cdt1 in Xenopus laevis.

Maiorano, D., Rul, W., and Marcel Mechali


+ Replication and Genome Dynamics

Specification of a DNA replication origin by a transcription complex.

Danis, E., Brodolin, K., Menut, S., Maiorano, D., Girard-Reydet, C. and Marcel Méchali.


+ Replication and Genome Dynamics

Repression of origin assembly in metaphase depends on inhibition of RLF-B/cdt1 by geminin.

Tada, S., Li, A., Maiorano, D., Méchali, M., and Blow, J.


+ Replication and Genome Dynamics

Stepwise Regulated Chromatin Assembly of MCM2-7 Proteins.

Maiorano, D., Lemaître, J.M. and Méchali, M.


+ Replication and Genome Dynamics

XCDT1 is required for the assembly of pre-replicative complexes in Xenopus laevis

Maiorano, D., Moreau, J., and Méchali, M.


+ Replication and Genome Dynamics

Initiation of DNA replication in eukaryotes : questioning the origin.

Françon, P., Maiorano, D. and Méchali, M.


+ Replication and Genome Dynamics

Evidence for different MCM subcomplexes with differential binding to chromatin in Xenopus.

Coué, M., Amariglio, F., Maiorano, D., Bocquet, S. and Méchali, M.


+ Replication and Genome Dynamics

SAKSOUK Nehme
SAKSOUK Nehme
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BELMEGUENAI Ramzi
BELMEGUENAI Ramzi
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ELOUALID Abdelmajid
ELOUALID Abdelmajid
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OUASS Sofian
OUASS Sofian
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SERHAL Kamar-Al-Zaman
SERHAL Kamar-Al-Zaman
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STEFANCIKOVA Lenka
STEFANCIKOVA Lenka
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MORENCY Éric
MORENCY Éric
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NAJAR Fatima
NAJAR Fatima
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SHARIF Samah
SHARIF Samah
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SOULET Caroline
SOULET Caroline
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PETRAI Stefano
PETRAI Stefano
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RECOLIN Benedicte
RECOLIN Benedicte
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MAISONNEUVE Dorothee
MAISONNEUVE Dorothee
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BOURAHLA Mohamed
BOURAHLA Mohamed
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GUILLAUME Carole
GUILLAUME Carole
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TSANOV Nikolay
TSANOV Nikolay
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ATGER Florian
ATGER Florian
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LANCIEN Amandine
LANCIEN Amandine
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DELUGIN Thomas
DELUGIN Thomas
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GOLFETTO Eleonora
GOLFETTO Eleonora
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MAZZI Wissam
MAZZI Wissam
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EL ETRI Jana
EL ETRI Jana
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HADDAD Yacine
HADDAD Yacine
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Subject: Molecular characterization of new factors involved in DNA damage tolerance
Hanane Mechri
- Start October 2021 Supervisor: Domenico Maiorano Co-supervisor: Antoine Aze

Thesis proposal: Subject: Molecular characterization of new factors involved in DNA damage tolerance