Purification and enrichment of specific chromatin loci.
Gauchier M, van Mierlo G, Vermeulen M, Déjardin J
Biology of Repetitive Sequences
Chromatin can be viewed as a highly complex mixture of proteins and nucleic acids that orchestrate DNA-based processes in the eukaryotic genome. Most of the mammalian genome is assembled into heterochromatin, a ‘closed’ structure imposed by several enzymatic activities. Such activities act on histones and the DNA itself to impinge on transcription, replication or repair.
Most of the heterochromatic fraction of the genome can be found at critical loci. These include telomeres, repetitive sequences around centromeres and a portion (about half) of the gene units encoding ribosomal RNAs. Defects in the regulation of these loci have therefore disastrous consequences on cell identity and can lead to developmental problems, cancer, premature aging or immune deficiencies. How precisely heterochromatic enzymes affect the composition of target loci has remained elusive and research in our laboratory primarily focuses on this question.
To understand how heterochromatin acts at the molecular level, we are looking at the effect of abrogating important heterochromatic activities, such as histone and/or DNA methyl-transferases, on the overall composition of key heterochromatic loci (telomeres, pericentromeres and rDNA).
Publications of the team
Telomeric Chromatin and TERRA.
Barral A, Déjardin J
Locus-specific chromatin isolation.
Vermeulen M, Déjardin J
Remodeling and destabilization of chromosome 1 pericentromeric heterochromatin by SSX proteins.
Traynor S, Møllegaard NE, Jørgensen MG, Brückmann NH, Pedersen CB, Terp MG, Johansen S, Dejardin J, Ditzel HJ, Gjerstorff MF
SMCHD1 is involved in de novo methylation of the DUX4-encoding D4Z4 macrosatellite.
Dion C, Roche S, Laberthonnière C, Broucqsault N, Mariot V, Xue S, Gurzau AD, Nowak A, Gordon CT, Gaillard MC, El-Yazidi C, Thomas M, Schlupp-Robaglia A, Missirian C, Malan V, Ratbi L, Sefiani A, Wollnik B, Binetruy B, Salort Campana E, Attarian S, Bernard R, Nguyen K, Amiel J, Dumonceaux J, Murphy JM, Déjardin J, Blewitt ME, Reversade B, Robin JD, Magdinier F
Integrative Proteomic Profiling Reveals PRC2-Dependent Epigenetic Crosstalk Maintains Ground-State Pluripotency.
van Mierlo G, Dirks RAM, De Clerck L, Brinkman AB, Huth M, Kloet SL, Saksouk N, Kroeze LI, Willems S, Farlik M, Bock C, Jansen JH, Deforce D, Vermeulen M, Déjardin J, Dhaenens M, Marks H
Telomere chromatin establishment and its maintenance during mammalian development.
Tardat M, Déjardin J
Histone H4K20 tri-methylation at late-firing origins ensures timely heterochromatin replication
Brustel J, Kirstein N, Izard F, Grimaud C, Prorok P, Cayrou C, Schotta G, Abdelsamie AF, Déjardin J, Méchali M, Baldacci G, Sardet C, Cadoret JC, Schepers A, Julien E.
Proteome Characterization of a Chromatin Locus Using the Proteomics of Isolated Chromatin Segments Approach
Kan SL, Saksouk N, Déjardin J
The molecular basis of the organization of repetitive DNA-containing constitutive heterochromatin in mammals
Nishibuchi G, Déjardin J.
The cell proliferation antigen Ki-67 organises heterochromatin
Sobecki M, Mrouj K, Camasses A, Parisis N, Nicolas E, Llères D, Gerbe F, Prieto S, Krasinska L, David A, Eguren M, Birling MC, Urbach S, Hem S, Déjardin J, Malumbres M, Jay P, Dulic V, Lafontaine DL, Feil RP, Fisher D
Switching between Epigenetic States at Pericentromeric Heterochromatin
Dejardin, J.
End-targeting proteomics of isolated chromatin segments of a mammalian ribosomal RNA gene promoter
Ide, S., Dejardin, J.
Constitutive heterochromatin formation and transcription in mammals
Saksouk N, Simboeck E, Déjardin J.
Nuclear-Receptor-Mediated Telomere Insertion Leads to Genome Instability in ALT Cancer
Marzec, P., Armenise, C., Perot, G., Roumelioti, F.M., Basyuk, E., Gagos, S., Chibon, F., Dejardin, J.
Redundant Mechanisms to Form Silent Chromatin at Pericentromeric Regions Rely on BEND3 and DNA Methylation
Saksouk N, Barth TK, Ziegler-Birling C, Olova N, Nowak A, Rey E, Mateos-Langerak J, Urbach S, Reik W, Torres-Padilla ME, Imhof A, Déjardin J.
How chromatin prevents genomic rearrangements: Locus colocalization induced by transcription factor binding.
Dejardin, J.
Thèses et hdr
SETDB1 and NSDs-dependent dual heterochromatin H3K9me3/H3K36me3 ensures gene expression by keeping enhancers inactive 07/07/2021
Defended by Amandine Barral on 07-07-2021