Maintenance of genome integrity during DNA replication

All research teams

The human body is made of 3.7x10¹³ cells, which contain about two meters of DNA each. As our cells undergo a total of 10¹⁶ divisions in a life time, they synthesize therefore more than 2x10¹⁶ meters of DNA, which represents 130,000 times the distance from Earth to the sun! This daunting task is executed by micro-machines called replisomes, containing hundreds of proteins. Replisomes assemble at replication origins and generate DNA structures called replication forks. As they progress along the chromosomes, replisomes often encounter obstacles such as DNA lesions or transcription complexes, leading to replication fork arrest. Stalled forks are fragile structures that can give rise to chromosome breaks and trigger genomic instability if they are not rapidly restarted. Fork stalling occurs even more frequently in cancer cells due to the deregulation of oncogenic pathways. Oncogene-induced replication stress (RS) promotes genomic instability and is therefore the driving force of tumorigenesis. However, RS represents also the Achilles’ heel of cancer cells as it interferes with cell proliferation and sensitizes them to chemotherapeutic agents. Understanding how normal and cancer cells respond to replication stress represents therefore a major challenge in cancer biology.

Our group investigates the cellular responses to replication stress in budding yeast and in human cell lines. Owing to the small size of its genome and the power of molecular genetics, budding yeast is an invaluable model organism to study the RS response and to characterize novel mechanisms that are conserved in human cells and are relevant to cancer biology. This is achieved through the use of powerful technologies to monitor the progression, arrest and recovery of replication forks. These methods include single-molecule approaches such as DNA combing and DNA fiber spreading, chromosome-based assays such as pulsed-field gel electrophoresis and NGS-based assays such as ChIP-seq, BrdU-IP-seq, DRIP-seq and BLESS. Together, these methods provide a comprehensive view of the replication stress response in yeast and human cells, from individual DNA molecules to whole genomes.

To further investigate the links between replication stress and cancer, we have recently teamed up with the group of Jérôme Moreaux (Hematology Department of the University Hospital of Montpellier) who is an expert of the pathophysiology of malignant plasma cells and in particular of Multiple Myeloma (MM).

LENGRONNE Armelle
Researcher

LIN Yea-Lih
Researcher

PARDO Benjamin
Researcher

PASERO Philippe
Director
Researcher

POLI Jérôme

Researcher

BARTHE Antoine
IT

TORáN Alba
IT

GOPAUL Diyavarshini
Postdoc

TECHER Herve
Postdoc

COURTES Mathilde
PhD Student

DA MOTA Mégane
PhD Student

HEUZE Jonathan
PhD Student

YANG Chun-Yen
PhD Student

LERAY Baptiste
Stagiaire

PRADEL Lea
Stagiaire

PUBLICATIONS OF THE TEAM

Toxic R-loops: Cause or consequence of replication stress?

Published the 03/08/2021 - DNA Repair

Samira Kemiha, Jérôme Poli, Yea-Lih Lin, Armelle Lengronne, Philippe Pasero

A Role for the Mre11-Rad50-Xrs2 Complex in Gene Expression and Chromosome Organization

Published the 04/12/2020 - Molecular cell

Forey R, Barthe A, Tittel-Elmer M, Wery M, Barrault MB, Ducrot C, Seeber A, Krietenstein N, Szachnowski U, Skrzypczak M, Ginalski K, Rowicka M, Cobb J, Rando OJ, Soutourina J, Dubrana K, Gasser SM, Morillon A, Pasero P, Lengronne A and Poli J

Topoisomerase 1 prevents replication stress at R-loop-enriched transcription termination sites

Published the 07/08/2020 - Nature Communications

Promonet A, Padioleau I, Liu Y, Sanz L, Biernacka A, Schmitz AL, Skrzypczak M, Sarrazin A, Mettling C, Rowicka M, Ginalski K, Chedin F, Chen CL, Lin YL, Pasero P

Homologous recombination and Mus81 promote replication completion in response to replication fork blockage

Published the 17/05/2020 - EMBO , 10.15252/embr.201949367

Benjamin Pardo, María Moriel‐Carretero, Thibaud Vicat, Andrés Aguilera and Philippe Pasero

Mec1 Is Activated at the Onset of Normal S Phase by Low-dNTP Pools Impeding DNA Replication

Published the 12/03/2020 - Molecular Cell

Romain Forey, Ana Poveda, Sushma Sharma, Antoine Barthe, Ismael Padioleau, Claire Renard, Robin Lambert, Magdalena Skrzypczak, Krzysztof Ginalski, Armelle Lengronne, Andrei Chabes, Benjamin Pardo, Philippe Pasero

MRX Increases Chromatin Accessibility at Stalled Replication Forks to Promote Nascent DNA Resection and Cohesin Loading.

Published the 25/11/2019 - Mol Cell , 77(2):395-410.e3 31759824

Delamarre A, Barthe A, de la Roche Saint-André C, Luciano P, Forey R, Padioleau I, Skrzypczak M, Ginalski K, Géli V, Pasero P, Lengronne A

Inhibition of Ataxia-Telangiectasia Mutated and RAD3-related (ATR) overcomes oxaliplatin resistance and promotes anti-tumor immunity in colorectal cancer.

Published the 17/04/2019 - Cancer Res 30987998

Combes E, Andrade AF, Tosi D, Michaud HA, Coquel F, Garambois V, Desigaud D, Jarlier M, Coquelle A, Pasero P, Bonnefoy N, Moreaux J, Martineau P, Del Rio M, Beijersbergen RL, Vezzio-Vie N, Gongora C

Overexpression of Claspin and Timeless protects cancer cells from replication stress in a checkpoint-independent manner.

Published the 24/02/2019 - Nat Commun , 10(1):910 30796221

Bianco JN, Bergoglio V, Lin YL, Pillaire MJ, Schmitz AL, Gilhodes J, Lusque A, Mazières J, Lacroix-Triki M, Roumeliotis TI, Choudhary J, Moreaux J, Hoffmann JS, Tourrière H, Pasero P

EZH2 is overexpressed in transitional preplasmablasts and is involved in human plasma cell differentiation.

Published the 14/02/2019 - Leukemia 30755708

Herviou L, Jourdan M, Martinez AM, Cavalli G, Moreaux J

DDR Inc., one business, two associates.

Published the 24/11/2018 - Curr Genet 30467717

Moriel-Carretero M, Pasero P, Pardo B

Myeloid-derived suppressor cells induce multiple myeloma cell survival by activating the AMPK pathway.

Published the 13/11/2018 - Cancer Lett , 442:233-241 30419344

De Veirman K, Menu E, Maes K, De Beule N, De Smedt E, Maes A, Vlummens P, Fostier K, Kassambara A, Moreaux J, Van Ginderachter JA, De Bruyne E, Vanderkerken K, Van Valckenborgh E

i-BLESS is an ultra-sensitive method for detection of DNA double-strand breaks.

Published the 06/11/2018 - Commun Biol , 1:181 30393778

Biernacka A, Zhu Y, Skrzypczak M, Forey R, Pardo B, Grzelak M, Nde J, Mitra A, Kudlicki A, Crosetto N, Pasero P, Rowicka M, Ginalski K

SAMHD1 and the innate immune response to cytosolic DNA during DNA replication.

Published the 08/10/2018 - Curr Opin Immunol , 56:24-30 30292848

Coquel F, Neumayer C, Lin YL, Pasero P

PRC2 targeting is a therapeutic strategy for EZ score defined high-risk multiple myeloma patients and overcome resistance to IMiDs.

Published the 05/10/2018 - Clin Epigenetics , 10(1):121 30285865

Herviou L, Kassambara A, Boireau S, Robert N, Requirand G, Müller-Tidow C, Vincent L, Seckinger A, Goldschmidt H, Cartron G, Hose D, Cavalli G, Moreaux J

Mrc1 and Rad9 cooperate to regulate initiation and elongation of DNA replication in response to DNA damage.

Published the 31/08/2018 - EMBO J 30158111

Bacal J, Moriel-Carretero M, Pardo B, Barthe A, Sharma S, Chabes A, Lengronne A, Pasero P

DDK Has a Primary Role in Processing Stalled Replication Forks to Initiate Downstream Checkpoint Signaling.

Published the 30/08/2018 - Neoplasia , 20(10):985-995 30157471

Sasi NK, Coquel F, Lin YL, MacKeigan JP, Pasero P, Weinreich M

Physiological and druggable skipping of immunoglobulin variable exons in plasma cells.

Published the 22/08/2018 - Cell Mol Immunol 30127381

Ashi MO, Srour N, Lambert JM, Marchalot A, Martin O, Le Noir S, Pinaud E, Ayala MV, Sirac C, Saulière J, Moreaux J, Cogné M, Delpy L

[EZH2 is therapeutic target for personalized treatment in multiple myeloma].

Published the 26/07/2018 - Bull Cancer , 105(9):804-819 30041976

Herviou L, Cavalli G, Moreaux J

SAMHD1 acts at stalled replication forks to prevent interferon induction.

Published the 20/04/2018 - Nature , 557(7703):57-61 29670289

Coquel F, Silva MJ, Técher H, Zadorozhny K, Sharma S, Nieminuszczy J, Mettling C, Dardillac E, Barthe A, Schmitz AL, Promonet A, Cribier A, Sarrazin A, Niedzwiedz W, Lopez B, Costanzo V, Krejci L, Chabes A, Benkirane M, Lin YL, Pasero P

DNMTi/HDACi combined epigenetic targeted treatment induces reprogramming of myeloma cells in the direction of normal plasma cells.

Published the 04/03/2018 - Br J Cancer , 118(8):1062-1073 29500406

Bruyer A, Maes K, Herviou L, Kassambara A, Seckinger A, Cartron G, Rème T, Robert N, Requirand G, Boireau S, Müller-Tidow C, Veyrune JL, Vincent L, Bouhya S, Goldschmidt H, Vanderkerken K, Hose D, Klein B, De Bruyne E, Moreaux J

Senataxin resolves RNA:DNA hybrids forming at DNA double-strand breaks to prevent translocations.

Published the 09/02/2018 - Nat Commun , 9(1):533 29416069

Cohen S, Puget N, Lin YL, Clouaire T, Aguirrebengoa M, Rocher V, Pasero P, Canitrot Y, Legube G

Dbf4 recruitment by forkhead transcription factors defines an upstream rate-limiting step in determining origin firing timing.

Published the 14/01/2018 - Genes Dev , 31(23-24):2405-2415 29330352

Fang D, Lengronne A, Shi D, Forey R, Skrzypczak M, Ginalski K, Yan C, Wang X, Cao Q, Pasero P, Lou H

Loss of RASSF4 Expression in Multiple Myeloma Promotes RAS-Driven Malignant Progression.

Published the 21/12/2017 - Cancer Res , 78(5):1155-1168 29259009

De Smedt E, Maes K, Verhulst S, Lui H, Kassambara A, Maes A, Robert N, Heirman C, Cakana A, Hose D, Breckpot K, van Grunsven LA, De Veirman K, Menu E, Vanderkerken K, Moreaux J, De Bruyne E

CD24, CD27, CD36 and CD302 gene expression for outcome prediction in patients with multiple myeloma.

Published the 13/12/2017 - Oncotarget , 8(58):98931-98944 29228738

Alaterre E, Raimbault S, Goldschmidt H, Bouhya S, Requirand G, Robert N, Boireau S, Seckinger A, Hose D, Klein B, Moreaux J

Nucleases Acting at Stalled Forks: How to Reboot the Replication Program with a Few Shortcuts

Published the 01/01/2017 - Annu Rev Genet. , 51:477-499 29178820

Pasero P, Vindigni A.

Signaling Pathways of Replication Stress in Yeast

Published the 01/01/2017 - FEMS Yeast Res. , 17, 2 27915243

Pardo B, Crabbé L, Pasero P

Nuclear DNA replication and repair in parasites of the genus Leishmania: Exploiting differences to develop innovative therapeutic approaches

Published the 01/01/2017 - Crit Rev Microbiol , 43, 2, 156-177 27960617

Uzcanga G, Lara E, Gutiérrez F, Beaty D, Beske T, Teran R, Navarro JC, Pasero P, Benítez W, Poveda A

RECQ1 helicase is involved in replication stress survival and drug resistance in multiple myeloma

Published the 01/01/2017 - Leukemia , 31, 10, 2104-2113 28186131

Viziteu E, Klein B, Basbous J, Lin YL, Hirtz C, Gourzones C, Tiers L, Bruyer A, Vincent L, Grandmougin C, Seckinger A, Goldschmidt H, Constantinou A, Pasero P, Hose D, Moreaux J.

Global miRNA expression analysis identifies novel key regulators of plasma cell differentiation and malignant plasma cell.

Published the 01/01/2017 - Nucleic Acids Res. , 45, 10, 5639-5652 28459970

Kassambara A, Jourdan M, Bruyer A, Robert N, Pantesco V, Elemento O, Klein B, Moreaux J.

Hypoxia favors the generation of human plasma cells

Published the 01/01/2017 - Cell Cycle , 1-14 28463531

Schoenhals M, Jourdan M, Bruyer A, Kassambara A, Klein B, Moreaux J.

Characterization of human FCRL4-positive B cells

Published the 01/01/2017 - PLoS One , 12(6):e0179793

Jourdan M, Robert N, Cren M, Thibaut C, Duperray C, Kassambara A, Cogné M, Tarte K, Klein B, Moreaux J.

Transcription-Replication Conflicts: Orientation Matters

Published the 01/01/2017 - CELL , 170(4):603-604 28802036

Lin, YL., Pasero, P.

Automated and simplified identification of normal and abnormal plasma cells in Multiple Myeloma by flow cytometry

Published the 01/01/2017 - Cytometry B Clin Cytom 28865180

Alaterre E, Raimbault S, Garcia JM, Rème T, Requirand G, Klein B, Moreaux J.

Extracellular S100A9 Protein in Bone Marrow Supports Multiple Myeloma Survival by Stimulating Angiogenesis and Cytokine Secretion.

Published the 01/01/2017 - Cancer Immunol Res , 5(10):839-846 28903971

De Veirman K, De Beule N, Maes K, Menu E, De Bruyne E, De Raeve H, Fostier K, Moreaux J, Kassambara A, Hose D, Heusschen R, Eriksson H, Vanderkerken K, Van Valckenborgh E.

Identifying high-risk adult AML patients: epigenetic and genetic risk factors and their implications for therapy

Published the 01/01/2016 - Expert Rev Hematol. , 9(4):351-60 26761438

Bret C, Viziteu E, Kassambara A, Moreaux J.

Inhibiting the anaphase promoting complex/cyclosome induces a metaphase arrest and cell death in multiple myeloma cells

Published the 01/01/2016 - Oncotarget. , 7(4):4062-76

Lub S, Maes A, Maes K, De Veirman K, De Bruyne E, Menu E, Fostier K, Kassambara A, Moreaux J, Hose D, Leleu X, King RW, Vanderkerken K, Van Valckenborgh E.

Mec1, INO80, and the PAF1 complex cooperate to limit transcription replication conflicts through RNAPII removal during replication stress

Published the 01/01/2016 - Genes Dev. , 30(3):337-54 26798134

Poli J, Gerhold CB, Tosi A, Hustedt N, Seeber A, Sack R, Herzog F, Pasero P, Shimada K, Hopfner KP, Gasser SM.

RECQ helicases are deregulated in hematological malignancies in association with a prognostic value

Published the 01/01/2016 - Biomark Res , 4, 3 26877874

Viziteu E, Kassambara A, Pasero P, Klein B, Moreaux J.

Chetomin, targeting HIF-1α/p300 complex, exhibits antitumour activity in multiple myeloma

Published the 01/01/2016 - Br J Cancer , 114(5):519-23 26867162

Viziteu E, Grandmougin C, Goldschmidt H, Seckinger A, Hose D, Klein B, Moreaux J.

Differential effects of lenalidomide during plasma cell differentiation

Published the 01/01/2016 - Oncotarget , 7(19):28096-111 27057635

Jourdan M, Cren M, Schafer P, Robert N, Duperray C, Vincent L, Ceballos P, Cartron G, Rossi JF, Moreaux J, Chopra R, Klein B

USP1 Regulates Cellular Senescence by Controlling Genomic Integrity

Published the 01/01/2016 - Cell Rep , 15, 7, 1401-1411 27160904

Ogrunc M, Martinez-Zamudio RI, Sadoun PB, Dore G, Schwerer H, Pasero P, Lemaitre JM, Dejean A, Bischof O

Forced KLF4 expression increases the generation of mature plasma cells and uncovers a network linked with plasma cell stage

Published the 01/01/2016 - Cell Cycle , 1, 10 27230497

Schoenhals M, Jourdan M, Seckinger A, Pantesco V, Hose D, Kassambara A, Moreaux J, Klein B.

Phosphorylation of CMG helicase and Tof1 is required for programmed fork arrest

Published the 01/01/2016 - Proc Natl Acad Sci U S A , 113(26):E3639-48. 27298353

Bastia D, Srivastava P, Zaman S, Choudhury M, Mohanty BK, Bacal J, Langston LD, Pasero P, O'Donnell ME

RPA Mediates Recruitment of MRX to Forks and Double-Strand Breaks to Hold Sister Chromatids Together

Published the 01/01/2016 - Mol. Cell , 64, 5, 951-966 27889450

Seeber A, Hegnauer AM, Hustedt N, Deshpande I, Poli J, Eglinger J, Pasero P, Gut H, Shinohara M, Hopfner KP, Shimada K, Gasser SM.

Drug metabolism and clearance system in tumor cells of patients with multiple myeloma

Published the 01/01/2015 - Oncotarget , 6(8):6431-47 25669983

Hassen W, Kassambara A, Reme T, Sahota S, Seckinger A, Vincent L, Cartron G, Moreaux J, Hose D, Klein B

In vivo treatment with epigenetic modulating agents induces transcriptional alterations associated with prognosis and immunomodulation in multiple myeloma

Published the 01/01/2015 - Oncotarget , 6(5):3319-34 25669970

Maes K, De Smedt E, Kassambara A, Hose D, Seckinger A, Van Valckenborgh E, Menu E, Klein B, Vanderkerken K, Moreaux J, De Bruyne E

Expression and role of RIP140/NRIP1 in chronic lymphocytic leukemia

Published the 01/01/2015 - J Hematol Oncol , 8(1):20 25879677

Lapierre M, Castet-Nicolas A, Gitenay D, Jalaguier S, Teyssier C, Bret C, Cartron G, Moreaux J, Cavaillès V. J Hematol Oncol. 2015 Mar 4;8(1):20.

miRNAs in multiple myeloma - a survival relevant complex regulator of gene expression

Published the 01/01/2015 - Oncotarget , 6(36):39165-39183 26472281

Seckinger A, Meißner T, Moreaux J, Benes V, Hillengass J, Castoldi M, Zimmermann J, Ho AD, Jauch A, Goldschmidt H, Klein B, Hose D.

Essential Roles of the Smc5/6 Complex in Replication through Natural Pausing Sites and Endogenous DNA Damage Tolerance

Published the 01/01/2015 - Mol. Cell , 60(6):835-46 26698660

Menolfi D, Delamarre A, Lengronne A, Pasero P, Branzei D.

New histone supply regulates replication fork speed and PCNA unloading

Published the 01/01/2014 - J. Cell Biol. , 204(1):29-43 24379417

Mejlvang J, Feng Y, Alabert C, Neelsen KJ, Jasencakova Z, Zhao X, Lees M, Sandelin A, Pasero P, Lopes M, Groth A.

Domain within the helicase subunit Mcm4 integrates multiple kinase signals to control DNA replication initiation and fork progression

Published the 01/01/2014 - Proc Natl Acad Sci U S A , 111(18):E1899-908 24740181

Sheu YJ, Kinney JB, Lengronne A, Pasero P, Stillman B.

The causes of replication stress and their consequences on genome stability and cell fate

Published the 01/01/2014 - Semin Cell Dev Biol. , 30C:154-164 24818779

Magdalou I, Lopez BS, Pasero P, Lambert SA

The histone deacetylases sir2 and rpd3 act on ribosomal DNA to control the replication program in budding yeast.

Published the 01/01/2014 - Mol Cell. , 54(4):691-697 24856221

Yoshida K, Bacal J, Desmarais D, Padioleau I, Tsaponina O, Chabes A, Pantesco V, Dubois E, Parrinello H, Skrzypczak M, Ginalski K, Lengronne A, Pasero P

Closing the MCM cycle at replication termination sites

Published the 01/01/2014 - EMBO Rep , 15(12):1226-7

Lengronne A, Pasero P.

Caught in the Act: R-Loops Are Cleaved by Structure-Specific Endonucleases to Generate DSBs

Published the 01/01/2014 - Mol Cell , 56, 6, 721-722 25526530

Lin YL, Pasero P.

Nucleotide-resolution DNA double-strand break mapping by next-generation sequencing

Published the 01/01/2013 - Nat Methods. , 10(4): 361-5 23503052

Crosetto N, Mitra A, Silva MJ, Bienko M, Dojer N, Wang Q, Karaca E, Chiarle R, Skrzypczak M, Ginalski K, Pasero P, Rowicka M, Dikic I

Rescuing stalled or damaged replication forks

Published the 01/01/2013 - Cold Spring Harb Perspect Biol. , 5, 5, a012815. 23637285

Yeeles JT, Poli J, Marians KJ, Pasero P.

DNA repair pathways in human multiple myeloma: Role in oncogenesis and potential targets for treatment.

Published the 01/01/2013 - Cell Cycle , 12, 17, 2760-2773 23966156

Gourzones-Dmitriev C, Kassambara A, Sahota S, Rème T, Moreaux J, Bourquard P, Hose D, Pasero P, Constantinou A, Klein B.

Time to Be Versatile: Regulation of the Replication Timing Program in Budding Yeast

Published the 01/01/2013 - J Mol Biol. , 425(23):4696-705 24076190

Yoshida K, Poveda A, Pasero P.

DNA polymerase η modulates replication fork progression and DNA damage responses in platinum-treated human cells

Published the 01/01/2013 - Sci Rep. , 3, 3277 24253929

Sokol AM, Cruet-Hennequart S, Pasero P, Carty MP.

DNA replication stress response involving PLK1, CDC6, POLQ, RAD51 and CLASPIN upregulation prognoses the outcome of early/mid-stage non-small cell lung cancer patients

Published the 01/01/2012 - Oncogenesis , 1:e30. doi: 10.1038/oncsis.2012.29 23552402

Allera-Moreau C, Rouquette I, Lepage B, Oumouhou N, Walschaerts M, Leconte E, Schilling V, Gordien K, Brouchet L, Delisle MB, Mazieres J, Hoffmann JS, Pasero P, Cazaux C.

Histone h3 lysine 56 acetylation and the response to DNA replication fork damage.

Published the 01/01/2012 - Mol Cell Biol , 32, 1, 154-172

Wurtele H, Kaiser GS, Bacal J, St-Hilaire E, Lee EH, Tsao S, Dorn J, Maddox P, Lisby M, Pasero P, Verreault A.

dNTP pools determine fork progression and origin usage under replication stress.

Published the 01/01/2012 - EMBO J. , 31, 4, 883-894, doi: 10.1038/emboj.2011.470. 22234185

Poli J, Tsaponina O, Crabbé L, Keszthelyi A, Pantesco V, Chabes A, Lengronne A, Pasero P.

Analysis of DNA replication profiles in budding yeast and mammalian cells using DNA combing

Published the 01/01/2012 - Methods , 57, 2, 149-157 22579803

Bianco JN, Poli J, Saksouk J, Bacal J, Silva MJ, Yoshida K, Lin YL, Tourrière H, Lengronne A, Pasero P.

Cohesin Association to Replication Sites Depends on Rad50 and Promotes Fork Restart.

Published the 01/01/2012 - Mol Cell. , 48, 1, 98-108. doi: 10.1016/j.molcel.2012.07.004 22885006

Tittel-Elmer M, Lengronne A, Davidson MB, Bacal J, François P, Hohl M, Petrini JH, Pasero P, Cobb JA.

Interference between DNA replication and transcription as a cause of genomic instability

Published the 01/01/2012 - Curr Genomics. , 13, 1, 65-73 22942676

Lin YL, Pasero P.

New Topoisomerase I mutations are associated with resistance to camptothecin

Published the 01/01/2011 - Mol. Cancer , 10, 1, 64 21619602

Gongora, C., Vezzio-Vie, N., Tuduri, S., Denis, V., Causse, A., Auzanneau, C., Collod-Beroud, G., Coquelle, A., Pasero, P., Pourquier, P., Martineau, P., Del Rio, M

Does interference between replication and transcription contribute to genomic instability in cancer cells?

Published the 01/01/2010 - Cell Cycle , 9, 10 20495385

Tuduri, S., Crabbe, L., Tourrière, H., Coquelle, A., Pasero, P.

Defining replication origin efficiency using DNA fiber assays.

Published the 01/01/2010 - Chromosome Res. , 18, 1, 91-102 20039120

Tuduri, S., Tourrière, H., Pasero, P.

The Smc5/6 complex is required for dissolution of DNA-mediated sister chromatid linkages

Published the 01/01/2010 - Nucleic Acids Res , 38, 19, 6502-6512 20571088

Bermúdez-López, M., Ceschia, A., de Piccoli, G., Colomina, N., Pasero, P., Aragón, L., Torres-Rosell, J.

Exo1 competes with repair synthesis, converts NER intermediates to long ssDNA gaps, and promotes checkpoint activation.

Published the 01/01/2010 - Mol. Cell. , 40, 1, 50-62 20932474

Giannattasio M, Follonier C, Tourrière H, Puddu F, Lazzaro F, Pasero P, Lopes M, Plevani P, Muzi-Falconi M

Analysis of replication profiles reveals key role of RFC-Ctf18 in yeast replication stress response.

Published the 01/01/2010 - Nat Struct Mol Biol , 17, 11 , 1391-1397. advance online publication 20972444

Crabbe, L., Thomas, A., Pantesco, V., De Vos, J., Pasero, P. and Lengronne, A.

Topoisomerase I suppresses genomic instability by preventing interference between replication and transcription

Published the 01/01/2010 - Nat. Cell Biol , 12, 11, 1122 21045805

Tuduri, S., Crabbé, L., Conti, C., Tourrière, H., Holtgreve-Grez, H., Jauch, A., Pantesco, V., De Vos, J., Thomas, A., Theillet, C., Pommier, Y., Tazi, J., Coquelle, A., Pasero, P

RNAi-based screening identifies the Mms22L-Nfkbil2 complex as a novel regulator of DNA replication in human cells

Published the 01/01/2010 - EMBO J , 29(24):4210-22 21113133

Piwko, W., Olma, MH., Held, M., Bianco, JN., Pedrioli, PG., Hofmann, K., Pasero, P., Gerlich, DW., Peter, M

Transcription and replication: Breaking the rules of the road causes genomic instability

Published the 01/01/2010 - Transcription , 1, 2, 99-102

Poveda, AM., Le Clech, M., Pasero, P.

Differential regulation of homologous recombination at DNA breaks and replication forks by the Mrc1 branch of the S-phase checkpoint.

Published the 01/01/2009 - EMBO J. , 28, 8, 1131-1141 19322196

Alabert C, Bianco JN, Pasero P

The MRX complex stabilizes the replisome independently of the S phase checkpoint during replication stress.

Published the 01/01/2009 - EMBO J. , 28(8):1142-56 19279665

Tittel-Elmer M, Alabert C, Pasero P, Cobb JA.

Specific function of phosphoinositide 3-kinase beta in the control of DNA replication.

Published the 01/01/2009 - Proc Natl Acad Sci U S A , 106, 18, 7525-7530 19416922

Marqués, M., Kumar, A., Poveda, AM., Zuluaga, S., Hernández, C., Jackson, S., Pasero, P., Carrera, AC.

Single-molecule analysis of DNA replication in yeast and in human cells.

Published the 01/01/2009 -

Alabert, C., Poveda, A. and Pasero, P

Topoisomerase I suppresses genomic instability by preventing interference between replication and transcription.

Published the 01/01/2009 - Nat Cell Biol. , 11, 11, 1315-1324 19838172

Tuduri S, Crabbé L, Conti C, Tourrière H, Holtgreve-Grez H, Jauch A, Pantesco V, De Vos J, Thomas A, Theillet C, Pommier Y, Tazi J, Coquelle A, Pasero P.

Involvement of a chromatin remodeling complex in damage tolerance during DNA replication

Published the 01/01/2009 - Nat. Struct. Mol. Biol , 16, 11, 1167-1172 19855395

Falbo, K.B., Alabert, C., Katou, Y., Wu, S., Han, J., Wehr, T., Xiao, J., He, X., Zhang, Z., Shi, Y., Shirahige, K., Pasero, P., Shen, X.

Defining replication origin efficiency using DNA fiber assays

Published the 01/01/2009 - Chromosome Res , 18, 1, 91-102 20039120

Tuduri, S., Tourrière, H., Pasero, P

A journey into the nucleus. Conference on Nuclear Structure and Dynamics.

Published the 01/01/2008 - EMBO Rep. , 9, 3, 228-233 18274551

Solovei, I., Pasero, P., Visa, N.

Rtt101 and Mms1 in budding yeast form a CUL4(DDB1)-like ubiquitin ligase that promotes replication through damaged DNA

Published the 01/01/2008 - EMBO Rep , 9, 10, 1034-1040 18704118

Zaidi, IW., Rabut, G., Poveda, A., Scheel, H., Malmström, J., Ulrich, H., Hofmann, K., Pasero, P., Peter, M., Luke, B.

Maintenance of fork integrity at damaged DNA and natural pause sites.

Published the 01/01/2007 - DNA Repair (Amst) , 6, 7, 900-913 17379579

Tourriere, H., Pasero Ph.

Anaphase onset before complete DNA replication with intact checkpoint responses

Published the 01/01/2007 - Science , 315, 5817, 1411-1415 17347440

Torres-Rosell J, De Piccoli G, Cordon-Preciado V, Farmer S, Jarmuz A, Machin F, Pasero P, Lisby M, Haber JE, Aragon L.

Upregulation of error-prone DNA polymerases beta and kappa slows down fork progression without activating the replication checkpoint

Published the 01/01/2007 - Cell Cycle , 6, 4, 471-477 17329970

Pillaire MJ, Betous R, Conti C, Czaplicki J, Pasero P, Bensimon A, Cazaux C, Hoffmann JS.

Phosphorylation of Slx4 by Mec1 and Tel1 regulates the single-strand annealing mode of DNA repair in budding yeast

Published the 01/01/2007 - Mol Cell Biol. , 27, 18, 6433-6445 17636031

Flott, S., Alabert, C., Toh, GW., Toth, R., Sugawara, N., Campbell, DG., Haber, JE., Pasero, P., Rouse, J.

The cullin Rtt101p promotes replication fork progression through damaged DNA and natural pause sites.

Published the 01/01/2006 - Curr Biol. , 16, 786-792 16631586

Luke, B, Versini, G, Jaquenoud, M, Zaidi, IW, Kurz, T, Pintard, L, Pasero, P, Peter, M.

An essential role for Orc6 in DNA replication through maintenance of pre-replicative complexes

Published the 01/01/2006 - EMBO J. , 25, 5150-5158 17053779

Semple JW, Da-Silva LF, Jervis EJ, Ah-Kee J, Al-Attar H, Kummer L, Heikkila JJ, Pasero P, Duncker BP.

Mrc1 and tof1 promote replication fork progression and recovery independently of Rad53

Published the 01/01/2005 - Molecular Cell , 19 (5), 699-706. 16137625

Tourriere, H., Versini, G., Cordon-Preciado, V., Alabert, C., Pasero, P.

PUBLICATIONS COMMUNES

MRX Increases Chromatin Accessibility at Stalled Replication Forks to Promote Nascent DNA Resection and Cohesin Loading.

Delamarre A, Barthe A, de la Roche Saint-André C, Luciano P, Forey R, Padioleau I, Skrzypczak M, Ginalski K, Géli V, Pasero P, Lengronne A
2019 - Mol Cell , 77(2):395-410.e3 31759824
Service porteur : Maintenance of genome integrity during DNA replication

EZH2 in normal hematopoiesis and hematological malignancies

Herviou L, Cavalli G, Cartron G, Klein B, Moreaux J.
2016 - Oncotarget , 7, 3, 284-296 26497210
Service porteur : Chromatin and cell biology

DNA repair in diffuse large B-cell lymphoma: a molecular portrait

Bret C, Klein B, Cartron G, Schved JF, Constantinou A, Pasero P, Moreaux J.
2015 - Br J Haematol. , 169(2):296-9 25369781
Service porteur : Genetic Instability and Cancer

SAMHD1 is mutated recurrently in chronic lymphocytic leukemia and is involved in response to DNA damage

Clifford R, Louis T, Robbe P, Ackroyd S, Burns A, Timbs AT, Colopy GW, Dreau H, Sigaux F, Judde JG, Rotger M, Telenti A, Lin YL, Pasero P, Maelfait J, Titsias M, Cohen DR, Henderson SJ, Ross M, Bentley D, Hillmen P, Pettitt A, Rehwinkel J, Knight SJ, Taylor JC, Crow YJ, Benkirane M, Schuh A.
2014 - BLOOD , 123(7):1021-31 24335234
Service porteur : Laboratory of Molecular Virology

A DNA repair pathway score predicts survival in human multiple myeloma: the potential for therapeutic strategy

Kassambara A, Gourzones-Dmitriev C, Sahota S, Rème T, Moreaux J, Goldschmidt H, Constantinou A, Pasero P, Hose D, Klein B
2014 - Oncotarget , 5(9):2487-98 24809299
Service porteur : Genetic Instability and Cancer

Genetic and epigenetic determinants of DNA replication origins, position and activation

Méchali M, Yoshida K, Coulombe P, Pasero P.
2013 - Curr Opin Genet Dev. , 23, 2, 124-131. 23541525
Service porteur : Replication and Genome Dynamics

A DNA replication signature of progression and negative outcome in colorectal cancer

Pillaire, M.J., Selves, J., Gordien, K., Gouraud, P.A., Gentil, C., Danjoux, M., Do, C., Negre, V., Bieth, A., Guimbaud, R., Trouche, D., Pasero, P., Méchali, M., Hoffmann, JS, and Cazaux, C
2010 - Oncogen , 29, 6, 876-887 19901968
Service porteur : Replication and Genome Dynamics