Non-coding RNA, epigenetics and genome stability

Genome dynamics

Over the past several years RNA-based immunity against genomic invaders has been discovered in all branches of life ranging from the CRISPR-Cas systems in bacteria to piRNAs in animals. piRNAs, in Drosophila germline cells are encoded by specific loci called piRNA clusters. These loci establish and maintain memory of “nonself ” by generating small guides to induce an adaptive defense against genomic parasites like transposable elements (TEs). At the heart of this defense mechanism resides a piRNA induced silencing complex (piRISC) which consists of a piRNA and its associated Argonaute protein partner. Within piRISC, the small RNA determines target specificity by base pairing, while its Argonaute partner governs effector activity. The mRNA can be either cleaved in the cytoplasm (PTGS) or bound in the nucleus leading to TGS.

Figure 1

Biogenesis and function of the piRNAs in Drosophila germline cells

In the lab, we are dissecting the biogenesis and the role of piRISC in Drosophila. We have demonstrated that the Argonaute Piwi protein is required during Drosophila embryonic development for the activation of piRNA clusters via the deposition of H3K9me3 repressive marks. Once established in the embryo, this epigenetic mark will be maintained in a Piwi-independent manner during the whole ovarian development.

Figure 1

For our studies we combine fly genetics, proteomics and deep sequencing approaches (small RNA-seq, RNA-seq, ChIP-seq), using molecular and cell biology tools (CRISPR/dCas9).

Severine CHAMBEYRON

Researcher

0434359949
Charlotte Grimaud
Charlotte GRIMAUD

Researcher

0434359944
Alain PELISSON
Researcher

0434359948
Mourdas Mohamed
Mourdas MOHAMED
IT

0434359944
Bruno MUGAT
Gestionnaire parc binoculaires et injection
IT

0434359949
Anais Lugagne
Anais LUGAGNE
PhD Student

0434359944
Marion Varoqui
Marion VAROQUI
PhD Student

0434359949

PUBLICATIONS OF THE TEAM

The Mi-2 nucleosome remodeler and the Rpd3 histone deacetylase are involved in piRNA-guided heterochromatin formation

Bruno Mugat, Simon Nicot, Carolina Varela-Chavez, Christophe Jourdan, Kaoru Sato, Eugenia Basyuk, François Juge, Mikiko C. Siomi, Alain Pélisson & Séverine Chambeyron

more

The somatic piRNA pathway controls germline transposition over generations.

Barckmann B, El-Barouk M, Pélisson A, Mugat B, Li B, Franckhauser C, Fiston Lavier AS, Mirouze M, Fablet M, Chambeyron S

Transposable Element Misregulation Is Linked to the Divergence between Parental piRNA Pathways in Drosophila Hybrids

Romero-Soriano V, Modolo L, Lopez-Maestre H, Mugat B, Pessia E, Chambeyron S, Vieira C, Garcia Guerreiro MP

Piwi Is Required during Drosophila Embryogenesis to License Dual-Strand piRNA Clusters for Transposon Repression in Adult Ovaries

Akkouche, A., Mugat, B., Barckmann, B., Varela-Chavez, C., Li, B., Raffel, R., Pelisson, A., Chambeyron, S.

Identification of misexpressed genetic elements in hybrids between Drosophila-related species

Lopez-Maestre H, Carnelossi EA, Lacroix V, Burlet N, Mugat B, Chambeyron S, Carareto CM, Vieira C

Aubergine iCLIP Reveals piRNA-Dependent Decay of mRNAs Involved in Germ Cell Development in the Early Embryo

Barckmann,B., Pierson,S., Dufourt, J., Papin, C., Armenise, C., Port, F., Grentzinger, T., Chambeyron, S., Baronian, G., Desvignes, JP., Curk, T., Simonelig, M.


+ mRNA Regulation and Development

MicroRNA-Dependent Transcriptional Silencing of Transposable Elements in Drosophila Follicle Cells

Mugat B, Akkouche A, Serrano V, Armenise C, Li B, Brun C, Fulga TA, Van Vactor D, Pélisson A, Chambeyron S

A user-friendly chromatographic method to purify small regulatory RNAs

Grentzinger T, Armenise C, Pelisson A, Brun C, Mugat B, Chambeyron S.

Fast and Accurate Method to Purify Small Noncoding RNAs from Drosophila Ovaries

Grentzinger T, Chambeyron S.

A user-friendly chromatographic method to purify small regulatory RNAs

Grentzinger T, Armenise C, Pelisson A, Brun C, Mugat B, Chambeyron S

Maternally deposited germline piRNAs silence the tirant retrotransposon in somatic cells

Akkouche A, Grentzinger T, Fablet M, Armenise C, Burlet N, Braman V, Chambeyron S. §, Vieira C§ (co-dernier auteur)

Epigenetics and transgenerational inheritance

Brasset E, Chambeyron S.

piRNA-mediated transgenerational inheritance of an acquired trait

Grentzinger T, Armenise C, Brun C, Mugat B, Serrano V, Pelisson A, Chambeyron S.

NICOT Simon
NICOT Simon
IGH  Tubulin code
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AKKOUCHE Abdou
AKKOUCHE Abdou
GRED Clermont-Ferrand
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EL BAROUK Marianne
EL BAROUK Marianne
Attachée de recherche clinique
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LI Blaise
LI Blaise
Institut Pasteur
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VARELA CHAVEZ Carolina
VARELA CHAVEZ Carolina
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ADIMI Dina
ADIMI Dina
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BARCKMANN Bridlin
BARCKMANN Bridlin
CHU de Montpellier
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ESPARZA Nayeli
ESPARZA Nayeli
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BASYUK Eugenia
BASYUK Eugenia
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SINAN Mohammed
SINAN Mohammed
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JOURDAN Christophe
JOURDAN Christophe
Université de Montpellier
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DA SILVA BIZOUARD Megane
DA SILVA BIZOUARD Megane
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SANTT Olivier
SANTT Olivier
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OBRERO Jaime Whale
OBRERO Jaime Whale
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ATEK Nezli
ATEK Nezli
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BONNETAIN Emilie
BONNETAIN Emilie
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NINIO Maryvonne
NINIO Maryvonne
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RIEUVILLENEUVE Fabien
RIEUVILLENEUVE Fabien
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ENJALBERT Sandrine
ENJALBERT Sandrine
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CAHAIS Vincent
CAHAIS Vincent
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CHANUT Aurelie
CHANUT Aurelie
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CHEVALIER Laure
CHEVALIER Laure
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CLERIS Guilhem
CLERIS Guilhem
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CORRADINI Nicoletta
CORRADINI Nicoletta
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CORRIGER Lise
CORRIGER Lise
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DJELILATE Naima
DJELILATE Naima
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DOREL Carine
DOREL Carine
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ELAAZZAOUI Jalal
ELAAZZAOUI Jalal
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GUILLEMIN Severine
GUILLEMIN Severine
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GUISGANT Myriam
GUISGANT Myriam
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HISPARD Florian
HISPARD Florian
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IFRAH Moustapha
IFRAH Moustapha
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KHOURY Maroun
KHOURY Maroun
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LAFONT Laurent
LAFONT Laurent
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LAGLAINE Ael
LAGLAINE Ael
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LAKBIR Rachid
LAKBIR Rachid
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LISSILLOUR Celine
LISSILLOUR Celine
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MOUYSSET Julien
MOUYSSET Julien
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NAUROY Corinne
NAUROY Corinne
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PASSERAT Julien
PASSERAT Julien
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ROBIN Stephanie
ROBIN Stephanie
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SAGNIER Sophie
SAGNIER Sophie
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SAVARY Etienne
SAVARY Etienne
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SYOMIN Boris
SYOMIN Boris
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VAN GURP Thomas
VAN GURP Thomas
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VIEILLECHAIZE Florence
VIEILLECHAIZE Florence
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WAUQUIER Fabien
WAUQUIER Fabien
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LING Suyun
LING Suyun
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BARRULL Anne-lucie
BARRULL Anne-lucie
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SERRANO Vincent
SERRANO Vincent
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GRENTZINGER Thomas
GRENTZINGER Thomas
ETH Zurich
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ARMENISE Claudia
ARMENISE Claudia
Fasteris, Life Science Genesupport Switzerland
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GERMON Charlotte
GERMON Charlotte
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STROBEL Katrin
STROBEL Katrin
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GUILLAUME Mickael
GUILLAUME Mickael
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